Title: Foliar Spraying of Nanoselenium lmproves the Nutritional Quality ofAlfalfa by Recruiting Benefcial Phyllosphere Bacteria andRegulating the Distribution and Translocation of Selenium

Authors: Pengbo Sun, Zhijun Wang,* Gentu Ge, Lin Sun, Jian Bao, Yichao Liu, Xingquan Yan, Jiawei Zhang, Yuhan Zhang, and Yushan Jia*

Journal: AGRICULTURAL AND FOOD CHEMISTRY

Impact factor: IF2025=6.2（中科院一区TOP）

Abstract: Nanoselenium shows potential trends in improving plant health and food quality. In this study, diferentconcentrations of nanoselenium were sprayed on the leaves of alfalfa. Compared to the control, nanoselenium (100 mg-L-!signifcantly increased SeMet and SelMeCys contents in the roots, stems, and leaves of alfalfa. Nanoselenium educedmalondialdehyde by modulating the glutamine synthetase-glutamate synthetase (Gs-GOGAT)cycle and activating antioxidantenzymes, including the ascorbate-glutathione (AsA-GSH) cycle, as well as enhancing photosynthesis, resulting in an increase in thealfalfa yield, crude protein, and relative feeding value. The biofortification of nanoselenium mainly changed the community structureof phylosphere bacteria by reulating metabolic pathways such as amino acid metabolism, carbohydrate metabolism, and membranetransport, among which Proteobacteria were more responsive to nanoselenium. In conclusion, nanoselenium wil enhancephotosynthesis, improve signaling molecules, and recruit beneficial bacteria to regulate the nutritional levels of alfalfa.

Linkage: https://doi.org/10.1021/acs.jafc.4c08958